| Filename | Total | Flagged As Poor Quality | Sequence Length | %GC |
|---|---|---|---|---|
| FCC21WPACXX-CHKPEI13070002_L6_1.fq.gz | 67,032,866 | 0 | 70-100 | 55 |
| FCC21WPACXX-CHKPEI13070002_L6_2.fq.gz | 67,032,866 | 0 | 70-100 | 55 |
| FCC21WPACXX-CHKPEI13070003_L7_1.fq.gz | 63,542,676 | 0 | 70-100 | 55 |
| FCC21WPACXX-CHKPEI13070003_L7_2.fq.gz | 63,542,676 | 0 | 70-100 | 56 |
| FCC229TACXX-CHKPEI13070001_L3_1.fq.gz | 55,762,854 | 0 | 70-100 | 54 |
| FCC229TACXX-CHKPEI13070001_L3_2.fq.gz | 55,762,854 | 0 | 70-100 | 55 |
| FCC2GPDACXX-CHKPEI13070004_L2_1.fq.gz | 76,201,274 | 0 | 70-100 | 55 |
| FCC2GPDACXX-CHKPEI13070004_L2_2.fq.gz | 76,201,274 | 0 | 70-100 | 55 |
| FCC2GPDACXX-CHKPEI13070005_L3_1.fq.gz | 76,021,945 | 0 | 70-100 | 55 |
| FCC2GPDACXX-CHKPEI13070005_L3_2.fq.gz | 76,021,945 | 0 | 70-100 | 55 |
| FCC2GPDACXX-CHKPEI13070006_L4_1.fq.gz | 68,811,191 | 0 | 70-100 | 55 |
| FCC2GPDACXX-CHKPEI13070006_L4_2.fq.gz | 68,811,191 | 0 | 70-100 | 55 |
| FCC2GPDACXX-CHKPEI13070007_L6_1.fq.gz | 64,940,945 | 0 | 70-100 | 55 |
| FCC2GPDACXX-CHKPEI13070007_L6_2.fq.gz | 64,940,945 | 0 | 70-100 | 56 |
Library Sizes ranged between 55,762,854 and 76,201,274 reads.
Read totals for each library. Duplicated reads are conventionally an high overestimate at this point.
Summary of FastQC flags for each parameter
Heatmap showing mean base qualities for each library
Heatmap showing mean sequence qualities for each library
Heatmap of summed base distributions along each read
GC Content Heatmap normalised to theoretical GC content in the Mmusculus Genome
GC Content Distributions for all reads showing theoretical GC content from the Mmusculus Genome
Total overrepresented sequences for each library
## The Overrepresented_sequences module was empty in FCC21WPACXX-CHKPEI13070002_L6_1_fastqc.zip
## The Overrepresented_sequences module was empty in FCC21WPACXX-CHKPEI13070003_L7_1_fastqc.zip
## The Overrepresented_sequences module was empty in FCC21WPACXX-CHKPEI13070003_L7_2_fastqc.zip
## The Overrepresented_sequences module was empty in FCC229TACXX-CHKPEI13070001_L3_1_fastqc.zip
## The Overrepresented_sequences module was empty in FCC2GPDACXX-CHKPEI13070004_L2_1_fastqc.zip
## The Overrepresented_sequences module was empty in FCC2GPDACXX-CHKPEI13070005_L3_1_fastqc.zip
## The Overrepresented_sequences module was empty in FCC2GPDACXX-CHKPEI13070006_L4_1_fastqc.zip
## The Overrepresented_sequences module was empty in FCC2GPDACXX-CHKPEI13070007_L6_1_fastqc.zip
| Sequence | Total | Present In | Overall Percentage | Max_Percentage | Possible_Source |
|---|---|---|---|---|---|
| TGCAGGTCACCGAAGATAGGCACGAACCAACACTAATAAGTCTCCCCCAC | 1,593,574 | 5 | 0.169% | 0.692% | No Hit |
| TGCAGGTGTTTACAAAACCTATCAGGCAAACCGAATCCAAGCCTTCTCAT | 779,838 | 5 | 0.083% | 0.306% | No Hit |
| TGCAGGTCACCGAAGATAGGCACAAACCAACACTAATAAGTCTCCCCCAC | 712,872 | 5 | 0.075% | 0.309% | No Hit |
| TGCAGGTCACCGAAGACAGGCACGTTATCAACACCAATAAGTCTCCCCAC | 304,747 | 4 | 0.032% | 0.153% | No Hit |
| TGCAGGGGACTTACAAGACCTATCAGGCAAACCGAATCCAAGCCTTCTCA | 166,831 | 2 | 0.018% | 0.148% | No Hit |
| TGCAGTATAGTATTCTAAAGAGTACATATCCCATAATTTCTTTATCCAGT | 144,237 | 2 | 0.015% | 0.116% | No Hit |
| TGNAGGTCACCGAAGATAGGCACGAACCAACACTAATAAGTCTCCCCCAC | 94,135 | 1 | 0.010% | 0.140% | No Hit |
| TGCAGGTCACCGAAGATAGGCACGAATCAACACCAATAAGCCTCCCCACA | 76,838 | 1 | 0.008% | 0.138% | No Hit |
| TGCAGGTGCTTACAAGCCCTACCAATCAGGCAAACCGAATCCAAGCCTTC | 58,296 | 1 | 0.006% | 0.105% | No Hit |
Universal Adapter Content
## The Kmer_Content module was missing from FCC21WPACXX-CHKPEI13070002_L6_1_fastqc.zip
## The Kmer_Content module was missing from FCC21WPACXX-CHKPEI13070002_L6_2_fastqc.zip
## The Kmer_Content module was missing from FCC21WPACXX-CHKPEI13070003_L7_1_fastqc.zip
## The Kmer_Content module was missing from FCC21WPACXX-CHKPEI13070003_L7_2_fastqc.zip
## The Kmer_Content module was missing from FCC229TACXX-CHKPEI13070001_L3_1_fastqc.zip
## The Kmer_Content module was missing from FCC229TACXX-CHKPEI13070001_L3_2_fastqc.zip
## The Kmer_Content module was missing from FCC2GPDACXX-CHKPEI13070004_L2_1_fastqc.zip
## The Kmer_Content module was missing from FCC2GPDACXX-CHKPEI13070004_L2_2_fastqc.zip
## The Kmer_Content module was missing from FCC2GPDACXX-CHKPEI13070005_L3_1_fastqc.zip
## The Kmer_Content module was missing from FCC2GPDACXX-CHKPEI13070005_L3_2_fastqc.zip
## The Kmer_Content module was missing from FCC2GPDACXX-CHKPEI13070006_L4_1_fastqc.zip
## The Kmer_Content module was missing from FCC2GPDACXX-CHKPEI13070006_L4_2_fastqc.zip
## The Kmer_Content module was missing from FCC2GPDACXX-CHKPEI13070007_L6_1_fastqc.zip
## The Kmer_Content module was missing from FCC2GPDACXX-CHKPEI13070007_L6_2_fastqc.zip
## No Kmers were detected by FastQC